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Image Search Results
Journal:
Article Title: Serosal Mesothelium Retains Vasculogenic Potential
doi: 10.1002/dvdy.21334
Figure Lengend Snippet: Growth factors induce epithelial–mesenchymal transition (EMT) and smooth muscle actin (SMA) expression in serosal mesothelial cultures. Explants were cultured for 5 days under various conditions and analyzed for expression of Wt1 and SMA. A–C: After culture in normal medium, many peripheral cells of explants are fibroblastic and express SMA but not Wt1 (arrowheads), while Wt1-positive cells are negative for SMA (yellow open arrowhead). D–F: Under serum-free (sf) conditions, few of the epithelial sheet cells of cultured explants express SMA, whereas its majority express Wt1. SMA-positive cells can be Wt1-positive (open arrowheads) and Wt1-negative (arrowheads). G–L: Similar results were obtained for explants grown in sf-medium supplemented with basic fibroblast growth factor (bFGF) or epithelial growth factor (EGF), with few SMA-positive cells in the epithelial sheets. M–O: In explants grown in sf-medium supplemented with platelet-derived growth factor-BB (PDGF-BB), many fibroblastic peripheral cells in the epithelial sheet express SMA (arrowheads), many of which coexpress Wt1 (open arrowheads). Scale bar = 200 μm.
Article Snippet: Growth Factors Growth factors used were recombinant human EGF (40 ng/ml; R&D systems, 236-EG),
Techniques: Expressing, Cell Culture, Derivative Assay
Journal:
Article Title: Serosal Mesothelium Retains Vasculogenic Potential
doi: 10.1002/dvdy.21334
Figure Lengend Snippet: Production of smooth muscle actin (SMA) -positive cells in cultures of serosal mesothelium in the presence of specific growth factors. Cultures were analyzed at 5 days of culture with anti-SMA and anti-Wt1 antibodies. The total number of SMA-positive cells at the periphery was determined for each group indicated. One-way analysis of variance analysis determined that the number of SMA-positive cells in control serum-containing cultures was not significantly different from cultures with serum-free (sf) medium supplemented with platelet-derived growth factor-BB (PDGF-BB). In contrast to differentiation in serum-containing conditions, the number of SMA-positive cells in sf-medium or sf-medium supplemented with epithelial growth factor (EGF) or basic fibroblast growth factor (bFGF) was significantly (P < 0.001) reduced.
Article Snippet: Growth Factors Growth factors used were recombinant human EGF (40 ng/ml; R&D systems, 236-EG),
Techniques: Control, Derivative Assay